Journal: iScience

Article Title: TLR2/NF-κB signaling in macrophage/microglia mediated COVID-pain induced by SARS-CoV-2 envelope protein

doi: 10.1016/j.isci.2024.111027

Figure Lengend Snippet:

Article Snippet: For in vitro study, the siRNA targeting TLR2 (siTLR2, 50 nM, KeyGen Biotech, Jiangsu, China) or nontargeting scrambled controls (scRNA, 50 nM, KeyGen Biotech, Jiangsu, China) was applied by Lipocat3000 transfection reagent (Aoqing Biotechnology, Beijing, China).

Techniques: Recombinant, Protein Extraction, Endotoxin Assay, Western Blot, Virus, Software, Real-time Polymerase Chain Reaction, Imaging

Journal: bioRxiv

Article Title: MicroRNA inhibition using antimiRs in acute human brain tissue sections

doi: 10.1101/2022.04.05.487136

Figure Lengend Snippet: (A) Sequences of hsa-miR-134-5p (22-mer) and ant-134 (16-mer) indicates perfect complementarity between the two. (B) Experimental set-up to treat acutely sectioned human brain specimens with antimiR. Sections were placed into small inserts with a permeable mesh at the bottom. Inserts were placed into individual wells of a standard 12- well plate and submerged into 4 mL normal ACSF containing ant-134 or scr at varying concentrations. The ACSF is each well was oxygenated using a syringe needle connected to a carbogen gas supply. This preparation was left for 24 hours at room temperature. (C) RT- qPCR shows robust dose-dependent knockdown of miR-134 after 24 hours (Kruskal-Wallis test with Dunn’s test for multiple comparisons). (D) For the viable doses of ant-134, we did not observe off-target inhibition of either miR-10, miR-129 or miR-132 (all Kruskal-Wallis test with Dunn’s multiple comparisons tests)..

Article Snippet: Specimens were incubated for 24 hours at room temperature in the presence of either an ASO antimiR targeting hsa-miR-134-5p (‘Ant-134’; Qiagen, Manchester, UK; catalogue number 339132: TGGTCAACCAGTCACA), or a scrambled (Scr) nontargeting control antimiR (‘Negative control A’; Qiagen; catalogue number 339136: TAACACGTCTATACGCCCA), at 0.1, 1 and 3 µM.

Techniques: Quantitative RT-PCR, Inhibition

Journal: Frontiers in Immunology

Article Title: Comprehensive investigation into the influence of glycosylation on head and neck squamous cell carcinoma and development of a prognostic model for risk assessment and anticipating immunotherapy

doi: 10.3389/fimmu.2024.1364082

Figure Lengend Snippet: The role of SMS in HNSCC. (A) SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (Si-1 and Si-2) (B) The expression of SMS in HNSCC tissue and normal tissue of patients. t-test was used to compare the expression of genes between normal and tumor. (C, D) The results of the plate cloning assay of SMS expression in NC group, HN-5 cells and UMSCC-47 cells following treatment with siRNA sequences (E, F) Scratch-wound healing assay depicted that a significantly slower wound healing rate was observed in cells with a decreased expression of SMS. (G, H) Transwell assay showed that downregulation of SMS expression inhibited the migration and invasion capacity of HNSCC cells. To demonstrate the accuracy and reproducibility of the results, all experiments were repeated in two HNSCC (HN-5, UMSCC-47) cell lines and all data were presented as the means ± SD of three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The SMS siRNA expression vector and the scrambled siRNA nontarget control were acquired from Genewiz (China).

Techniques: Expressing, Clone Assay, Wound Healing Assay, Transwell Assay, Migration